Influence of subcellular fractions of mammalian testes on the mutagenic activity of nitrofurans toward Escherihia coli: Presence of a co-mutagen-like factor
作者： P. Maier , R.M. Philpot , G.R. Mohn , H.V. Malling
DOI: 10.1016/0027-5107(79)90056-3
关键词: Nitrofuran 、 Mutation 、 Biology 、 Microsome 、 Metabolism 、 Strain (chemistry) 、 Reversion 、 Biochemistry 、 Mutagen 、 Mutation frequency
摘要: Abstract The activation of nitrofurans to mutagenic intermediates by testicular tissue was investigated. AF-2 and nitrofurazone were tested in a microsomal suspension assay with strain E. coli K-12 343/113 as indicator subcellular fractions from rabbit testes. Different mutation patterns observed the presence or absence homogenate, indicating different intermediates. frequency arg + reversion increased proportionally homogenate concentration suggesting that activated components induced base-pair substitutions. Other experiments showed component low molecular weight, present soluble fraction homogenates testes rabbits, rats monkeys, responsible for frequency. It is concluded this “co-mutagen-like” factor either alters metabolism and/or promotes formation substitution-type mutations. This direct interaction between non-enzymic mammalian induction/expression process suggests role co-mutagen-like factors sensitivity nitrofurans.
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