Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control.

摘要: Two novel protein kinase C (PKC)-like genes, pck1+ and pck2+ were isolated from fission yeast by PCR. Both contain common domains of PKC-related molecules, but lack a putative Ca(2+)-binding domain so that they may belong to the nPKC group. Gene disruption establishes share an overlapping essential function for cell viability. Cells single pck2 deletion display severe defects in shape; are irregular sometimes pear-like instead cylindrical. In contrast, induced overexpression is lethal, producing multiseptated branched cells. These results suggest PKC-like genes involved polarity growth control. We show allelic sts6, locus we have previously identified its supersensitivity staurosporine, potent inhibitor [Toda et al. (1991) Genes Dev., 5, 60-73]. addition, lethal can be suppressed indicating pck1 molecular targets this inhibitor.