A combined small-angle X-ray and neutron scattering study of the structure of purified soluble gastrointestinal mucins.
作者： Pantelis Georgiades , Emanuela di Cola , Richard K. Heenan , Paul D. A. Pudney , David J. Thornton
DOI: 10.1002/BIP.22523
关键词: X-ray crystallography 、 Polymer 、 Small-angle X-ray scattering 、 Chemistry 、 Neutron diffraction 、 Analytical chemistry 、 Solubility 、 Neutron scattering 、 Scattering 、 Crystallography 、 Neutron
摘要: The structures of purified soluble porcine gastric (Muc5ac) and duodenal (Muc2) mucin solutions at neutral acidic pH were examined using small-angle X-ray scattering neutron experiments. We provide evidence for the morphology network above semidilute overlap concentration entanglement concentration. Furthermore, we investigated gelation both types in response to a reduction pH, where observed formation large-scale heterogeneities within polymer solutions, typical microphase-separated gels. dependence inhomogeneity length scale (Ξ) amplitude excess intensity [Iex(0)] are consistent with previously studied gelled synthetic polymeric systems. persistence lengths chains found be similar Muc5ac Muc2 from Kratky plots data (8 ± 2 nm). © 2014 Wiley Periodicals, Inc. Biopolymers 101: 1154–1164, 2014.
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