Pseudomonas aeruginosa attachment and biofilm development in dynamic environments

摘要: Summary Biofilm formation by Pseudomonas aeruginosa is hypothesized to follow a developmental pattern initiated attachment surface followed microcolony and mature biofilm development. Swimming twitching motility are important for development in P. aeruginosa. However, it clear that many strains lacking swimming exist as biofilms the lungs of cystic fibrosis patients. Consequently, we have developed dynamic assay identify motility-independent attachment-defective mutants. Using transposon mutagenesis, identified 14 novel attachment-deficient (dad) mutants including four specific conditions (dad specific). Two dad-specific contain insertions genes involved sensing responding external stimuli, implying significant impact factors on pathway. Observations initial long-term characterized our dad into two distinct classes: delayed impaired. Biofilm-delayed form wild-type but at least 24 h compared with wild type, whereas biofilm-impaired never assays. We propose model these classes.