The evaluation of the rates of biological processes from tracer kinetic data

摘要: Abstract Because messenger RNA continuously breaks down to form nucleotides that are reutilized for synthesis of all forms RNA, analysis the kinetics uptake precursors into nucleic acids cells is complicated. Our theoretical work ( Koch, 1962 ) now extended evaluate effects recycling case growing cells. Equations derived several different assumptions about turnover intermediate pool precursors. Even if one assumes infinitesimal, equations apply at times greater than a minute Escherichia coli. The effective rate constant smaller true by factor which ratio molecules enter from exogenous source total sources. initial lag in incorporation isotope can be used measure velocity sources size known. If unknown, it may estimated time extrapolated measurements times. Short times, however, do not necessarily reflect small pool. large and communicate with bypass or external medium but still give rise short because rapid precursor messenger. percentage particular classes acid relative quantity measured. These both amount labile