Comparative Proteome Analysis of hAT-MSCs Isolated from Chronic Renal Failure Patients with Differences in Their Bone Turnover Status

摘要: The relationship between the stem cells and bone turnover in uremic disease due to chronic renal failure (CRF) is not described. aim of this study was investigate effect status on cell properties. To search for presence such link shed some light stem-cell relevant mechanisms turnover, we carried out a with mesenchymal cells. Tissue biopsies were taken from abdominal subcutaneous adipose tissue CRF patient secondary hyperparathyroidism high disease. This underwent parathyroidectomy operation (PTX) another sample after PTX. A adynamic low healthy control also included. Mesenchymal isolated subjects analyzed using proteomic molecular approaches. Except ALP activity, did affect common However, detailed proteome analysis revealed regulated protein spots. total 32 spots identified following 2D gel electrophoresis MALDI-TOF/TOF analyzes. proteins classified into seven distinct groups their potential discussed. Distinct expression patterns emerged relation indicate possible CRF.