Translational control of the rat angiotensin type 1a receptor by alternative splicing

摘要: Abstract The rat angiotensin type 1a receptor (AT1aR) is comprised of three exons. Two transcripts are possible due to alternative splicing exon 2 (E1,3 and E1,2,3). Both code for identical AT1aR proteins since they differ only in the length their 5′ leader sequence (5′LS). We investigated functional differences these two stably transfected Chinese hamster ovary (CHO) cells also determined splice variant composition tissues. E1,3 expressing exhibited 1.8-fold higher AT1R densities five-fold levels Ang II-stimulated inositol phosphate production compared E1,2,3 cells. No mRNA or stability were seen. In vitro translation assays revealed protein from transcripts, suggesting reduces expression by inhibiting translation. Deletion 10 nucleotides increased mutated transcript which indistinguishable E1,3, that this loop region a predicted hairpin contributes inhibitory RNA cis element within 2. Comparison exonic tissues suggests regulated tissue-specific manner density.