Hand-powered centrifugal microfluidic platform inspired by the spinning top for sample-to-answer diagnostics of nucleic acids.
作者： Lu Zhang , Fei Tian , Chao Liu , Qiang Feng , Tingxuan Ma
DOI: 10.1039/C7LC01234A
关键词: Nucleic acid 、 Nucleic acid quantitation 、 Loop-mediated isothermal amplification 、 Computer hardware 、 Spinning 、 Nucleic acid amplification technique 、 Centrifuge 、 Computer science 、 Sample (material) 、 Microfluidics
摘要: Point-of-care (POC), sample-to-answer and electricity-free nucleic acid diagnostic tools are vital for health care disease control in resource-limited settings where centralized medical facilities or even electric power may remain unreliable. Inspired by one of the oldest recognizable toys, spinning top, here we report a fully hand-powered centrifugal microfluidic platform diagnostics pathogenic bacteria. Assay procedures such as zeolite-based purification acids, loop-mediated isothermal amplification (LAMP) visual detection fluorescence signals integrated into single disc. A simple pull-out operation top rack customized centrifuge initiates high-speed rotation disc, resulting efficient actuation mixing preloaded sample/reagent fluids. This enables simultaneous six kinds bacteria within small disc an manner, showing great promise remote settings.
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sci-hub.se 参考文章(56)
Maowei Dou, Sharma Timilsina Sanjay, Merwan Benhabib, Feng Xu, XiuJun Li, None, Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms Talanta. ,vol. 145, pp. 43- 54 ,(2015) , 10.1016/J.TALANTA.2015.04.068
Wenjing Lu, Jidong Wang, Qiong Wu, Jiashu Sun, Yiping Chen, Lu Zhang, Chunsheng Zheng, Wenna Gao, Yi Liu, Xingyu Jiang, High-throughput sample-to-answer detection of DNA/RNA in crude samples within functionalized micro-pipette tips Biosensors and Bioelectronics. ,vol. 75, pp. 28- 33 ,(2016) , 10.1016/J.BIOS.2015.08.016
Seung Jun Oh, Byung Hyun Park, Jae Hwan Jung, Goro Choi, Doh C. Lee, Do Hyun Kim, Tae Seok Seo, Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection. Biosensors and Bioelectronics. ,vol. 75, pp. 293- 300 ,(2016) , 10.1016/J.BIOS.2015.08.052
Sharma T. Sanjay, Guanglei Fu, Maowei Dou, Feng Xu, Rutao Liu, Hao Qi, XiuJun Li, Biomarker detection for disease diagnosis using cost-effective microfluidic platforms Analyst. ,vol. 140, pp. 7062- 7081 ,(2015) , 10.1039/C5AN00780A
Li Ren, Jian-Chun Wang, Wenming Liu, Qin Tu, Rui Liu, Xueqin Wang, Juan Xu, Yaolei Wang, Yanrong Zhang, Li Li, Jinyi Wang, An enzymatic immunoassay microfluidics integrated with membrane valves for microsphere retention and reagent mixing. Biosensors and Bioelectronics. ,vol. 35, pp. 147- 154 ,(2012) , 10.1016/J.BIOS.2012.02.034
T. Kawai, K. Tsutsumi, Adsorption characteristics of surfactants and phenol on modified zeolites from their aqueous solutions Colloid and Polymer Science. ,vol. 273, pp. 787- 792 ,(1995) , 10.1007/BF00658758
Wataru Yamazaki, Masanori Ishibashi, Ryuji Kawahara, Kiyoshi Inoue, Development of a loop-mediated Isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus BMC Microbiology. ,vol. 8, pp. 163- 163 ,(2008) , 10.1186/1471-2180-8-163
Qian-Jin Zhou, Lei Wang, Jiong Chen, Rui-Na Wang, Yu-Hong Shi, Chang-Hong Li, De-Min Zhang, Xiao-Jun Yan, Yan-Jun Zhang, None, Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals Journal of Microbiological Methods. ,vol. 104, pp. 26- 35 ,(2014) , 10.1016/J.MIMET.2014.06.008
Yukiko Hara-Kudo, Manabu Yoshino, Tadashi Kojima, Masanari Ikedo, Loop-mediated isothermal amplification for the rapid detection of Salmonella Fems Microbiology Letters. ,vol. 253, pp. 155- 161 ,(2005) , 10.1016/J.FEMSLE.2005.09.032
Eric K. Sackmann, Anna L. Fulton, David J. Beebe, The present and future role of microfluidics in biomedical research Nature. ,vol. 507, pp. 181- 189 ,(2014) , 10.1038/NATURE13118