Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals
作者： Qian-Jin Zhou , Lei Wang , Jiong Chen , Rui-Na Wang , Yu-Hong Shi
DOI: 10.1016/J.MIMET.2014.06.008
关键词: Vibrio anguillarum 、 Vibrio alginolyticus 、 Pathogenic bacteria 、 Biology 、 Vibrio harveyi 、 Vibrio vulnificus 、 Vibrio fluvialis 、 Vibrio parahaemolyticus 、 Microbiology 、 Loop-mediated isothermal amplification
摘要: … -chip LAMP. First, the serially diluted gDNA of each bacterium was analyzed by in-tube LAMP to evaluate the LOD of the primers before on-chip … that of the on-chip LAMP assay and the …
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