Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.
作者： E Paoletti , B R Lipinskas
DOI: 10.1128/JVI.26.3.822-824.1978
关键词: Enzyme 、 Endoribonuclease activity 、 Ribonucleoside 、 Biochemistry 、 Molecular biology 、 RNA 、 Polyadenylation 、 Messenger RNA 、 Biology 、 Virus 、 Endoribonuclease 、 Immunology 、 Insect Science 、 Microbiology 、 Virology
摘要: A soluble endoribonuclease activity was extracted from purified vaccinia virus cores by treatment with sodium-deoxycholate and dithiothreitol. The enzyme readily cleaved virion-associated high-molecular-weight RNA to limit-sized fragments sedimenting at 8 12S. Purified virion-released 12S polyadenylated mRNA not degraded the extract. did require presence of ribonucleoside triphosphates for activity.
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