Gene-transfer, stability, and biochemical properties of animal cells transformed with vaccinia DNA.

作者: Angel Pellicer , Mariano Esteban

DOI: 10.1016/0042-6822(82)90236-7

关键词: DNAGenomeBiologySouthern blotThymidine kinaseVacciniaMolecular biologyGeneVirologyCellVirus

摘要: Abstract The introduction and maintenance of the large DNA (180 kb) from vaccinia virus into animal cells has been accomplished by gene transfer techniques. While all or nearly genome could be found in some clones, no cytopathic effects were observed, indicating that was a noninfectious molecule. In addition, thymidine kinase (tk) unable to complement mutant phenotype mouse LtK(−) cells. arrangement viral sequences transformed analyzed Southern blots with clones maintained culture 50 200 cell generations. Some appear integrated high-molecular-weight cellular DNA. Using cloned terminal fragment as probe, we have obtained evidence which indicates free molecules are present number transformants. A biological properties these transformants analyzed. most significant observation failure support replication exogenously added while VSV Mengo viruses unaffected. This interference-like phenomenon is correlated presence

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