Differentiation of smooth muscle cells in the fetal rat testis and ovary: localization of alkaline phosphatase, smooth muscle myosin, F-actin, and desmin.

摘要: The histochemical demonstration of alkaline phosphatase (AP) activity and localization smooth muscle myosin (SMM), F-actin, desmin were carried out on frozen sections testes ovaries from 15-day-old fetal to newborn rats. presence immunocytochemically localized SMM was confirmed by Western blot analysis proteins isolated gonads. development cells predominant in the testis. first SMM-positive with an increasing intensity for F-actin appeared testicular tunica albuginea around cords age 16 days. A continuous layer SMM- F-actin-positive (but not uniformly desmin-positive) myoid detected In early gonads ovary, a majority interstitial expressed desmin, indicating that, undifferentiated tissues, non-myogenic may also express desmin. During development, male female gonocytes showed decrease content but retained their high AP activity. cortex rat observed be associated postnatal histogenesis follicles. SMM-containing hilus ovary required demarcation mesonephros constriction mesovarium. occurrence predominantly fetuses suggests that contractile testis induced androgens.