Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers.
作者： G.F. Cui , L.F. Wu , X.N. Wang , W.J. Jia , Q. Duan
关键词: Hybrid 、 Genetic marker 、 Genetic diversity 、 Cultivar 、 Principal component analysis 、 Genetic relationship 、 Genetic variation 、 Biology 、 Botany 、 Dendrogram
摘要: Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average 13 amplified by each primer. Two software packages, POPGENE 1.32 NTSYSpc 2.1, analyze the data matrix. Our results that observed number alleles (NA), effective (NE), Nei's genetic diversity (H), Shannon's information index (I) 1.9630, 1.4179, 0.2606, 0.4080, respectively. The highest similarity (0.9601) was between Oriental x Trumpet lilies, indicated two hybrids had a close relationship. An unweighted pair-group method with arithmetic means dendrogram clustered into discrete groups. first group included OT cultivars, while Asiatic, LA, Longiflorum lilies placed second cluster. distribution individuals principal component analysis consistent clustering dendrogram. Fingerprints built from 8 could be separated completely. This study confirmed effect efficiency identification cultivars.
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