Identification of miRNA, lncRNA and mRNA-associated ceRNA networks and potential biomarker for MELAS with mitochondrial DNA A3243G mutation.

摘要: Researchers in the field of mitochondrial biology are increasingly unveiling complex mechanisms between dysfunction and noncoding RNAs (ncRNAs). However, roles ncRNAs underlying myopathy remain unexplored. The aim this study was to elucidate regulating networks dysregulated Mitochondrial myopathy, Encephalopathy, Lactic Acidosis, Stroke-like episodes (MELAS) with DNA (mtDNA) A3243G mutation, which might make contributions and, possibly, new tools applicable clinical practice. Through high-throughput technology followed by quantitative real-time polymerase chain reaction (qRT-PCR) bioinformatics analyses, for first time, we found that muscle miRNAs lncRNAs 20 MELAS patients mtDNA mutation controls formed regulation participated immune system, signal transduction, translation, contraction other pathways discovery training phase. Then, selected were validated serum independent validation cohorts qRT-PCR. Finally, ROC curve analysis indicated reduced miR-27b-3p had better diagnosis value than lactate serve as a novel, noninvasive biomarker MELAS. Follow-up investigation is warranted understand pathogenesis.