AKT2 siRNA delivery with amphiphilic-based polymeric micelles show efficacy against cancer stem cells.
作者： Diana Rafael , Petra Gener , Fernanda Andrade , Joaquin Seras-Franzoso , Sara Montero
DOI: 10.1080/10717544.2018.1461276
关键词: Polyethylenimine 、 AKT2 、 Carcinogenesis 、 Gene delivery 、 Chemistry 、 Oncogene 、 Cancer stem cell 、 RNA interference 、 Cancer research 、 Gene silencing
摘要: Development of RNA interference-based therapies with appropriate therapeutic window remains a challenge for advanced cancers. Because cancer stem cells (CSC) are responsible sustaining the metastatic spread disease to distal organs and progressive gain resistance cancers, new anticancer should be validated specifically this subpopulation cells. A amphihilic-based gene delivery system that combines Pluronic® F127 micelles polyplexes spontaneously formed by electrostatic interaction between anionic siRNA cationic polyethylenimine (PEI) 10K, was designed (PM). Resultant PM gather requirements an efficient safe transport in terms its physicochemical characteristics, internalization capacity, toxicity profile silencing efficacy. were loaded against AKT2, important oncogene involved breast tumorigenesis, special role CSC malignancy. Efficacy siAKT2-PM isol...
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