SERCA1 truncated proteins unable to pump calcium reduce the endoplasmic reticulum calcium concentration and induce apoptosis.
作者： Mounia Chami , Devrim Gozuacik , David Lagorce , Marisa Brini , Pierre Falson
关键词: Calcium 、 SERCA 、 Molecular biology 、 Biology 、 Calcium signaling 、 Cytosol 、 Calcium-Transporting ATPases 、 Cell biology 、 Endoplasmic reticulum 、 Western blot 、 Calcium ATPase
摘要: By pumping calcium from the cytosol to ER, sarco/endoplasmic reticulum ATPases (SERCAs) play a major role in control of signaling. We describe two SERCA1 splice variants (S1Ts) characterized by exon 4 and/or 11 splicing, encoding COOH terminally truncated proteins, having only one seven calcium-binding residues, and thus unable pump calcium. As shown semiquantitative RT-PCR, S1T transcripts are differentially expressed several adult fetal human tissues, but not skeletal muscle heart. proteins expression was detected Western blot nontransfected cell lines. In transiently transfected cells, homodimers were revealed using mildly denaturing conditions. shown, confocal scanning microscopy, colocalize with endogenous SERCA2b into ER membrane. Using ER-targeted aequorin (erAEQ), we have found that reduce reverse elevation loading induced SERCA2b. Our results also show increase leakage consistent hypothesis cation channel formed homodimers. Finally, when overexpressed liver-derived significantly induce apoptosis. These data reveal further mechanism modulating Ca2+ accumulation nonmuscle cells highlight relevance
rupress.org
core.ac.uk
jcb.org参考文章(56)
M. Montero, M. Brini, R. Marsault, J. Alvarez, R. Sitia, T. Pozzan, R. Rizzuto, Monitoring dynamic changes in free Ca2+ concentration in the endoplasmic reticulum of intact cells. The EMBO Journal. ,vol. 14, pp. 5467- 5475 ,(1995) , 10.1002/J.1460-2075.1995.TB00233.X
H de Smedt, J A Eggermont, F Wuytack, J B Parys, L Van den Bosch, L Missiaen, J Verbis, R Casteels, Isoform switching of the sarco(endo)plasmic reticulum Ca2+ pump during differentiation of BC3H1 myoblasts. Journal of Biological Chemistry. ,vol. 266, pp. 7092- 7095 ,(1991) , 10.1016/S0021-9258(20)89614-8
Rosario Rizzuto, Marisa Brini, Carlo Bastianutto, Robert Marsault, Tullio Pozzan, [30] Photoprotein-mediated measurement of calcium ion concentration in mitochondria of living cells Methods in Enzymology. ,vol. 260, pp. 417- 428 ,(1995) , 10.1016/0076-6879(95)60155-4
Biermann Ja, Preston Ga, Murphy E, Barrett Jc, Effects of alterations in calcium homeostasis on apoptosis during neoplastic progression. Cancer Research. ,vol. 57, pp. 537- 542 ,(1997)
J Lytton, M Westlin, S.E. Burk, G.E. Shull, D.H. MacLennan, Functional comparisons between isoforms of the sarcoplasmic or endoplasmic reticulum family of calcium pumps. Journal of Biological Chemistry. ,vol. 267, pp. 14483- 14489 ,(1992) , 10.1016/S0021-9258(19)49738-X
Sophie Launay, Maurizio Giannı̀, Tünde Kovàcs, Raymonde Bredoux, Arlette Bruel, Pascal Gélébart, Fabien Zassadowski, Christine Chomienne, Jocelyne Enouf, Béla Papp, Lineage-specific modulation of calcium pump expression during myeloid differentiation. Blood. ,vol. 93, pp. 4395- 4405 ,(1999) , 10.1182/BLOOD.V93.12.4395.412K06_4395_4405
JB Michael, DB Martin, L Peter, Calcium - a life and death signal Nature. ,vol. 395, pp. 645- 648 ,(1998) , 10.1038/27094
Yuzo Furuya, Per Lundmo, Alison D Short, Donald L Gill, John T Isaacs, None, The role of calcium, pH, and cell proliferation in the programmed (apoptotic) death of androgen-independent prostatic cancer cells induced by thapsigargin. Cancer Research. ,vol. 54, pp. 6167- 6175 ,(1994)
Marisa Brini, Robert Marsault, Carlo Bastianutto, Javier Alvarez, Tullio Pozzan, Rosario Rizzuto, Transfected Aequorin in the Measurement of Cytosolic Ca2+ Concentration ([Ca2+]c) A CRITICAL EVALUATION Journal of Biological Chemistry. ,vol. 270, pp. 9896- 9903 ,(1995) , 10.1074/JBC.270.17.9896
Jacopo Meldolesi, Tullio Pozzan, The endoplasmic reticulum Ca2+ store: a view from the lumen Trends in Biochemical Sciences. ,vol. 23, pp. 10- 14 ,(1998) , 10.1016/S0968-0004(97)01143-2