The Anti-apoptotic Effect of Polypeptide from Chlamys farreri (PCF) in UVB-Exposed HaCaT Cells Involves Inhibition of iNOS and TGF-β1

摘要: To investigate the molecular mechanisms of polypeptide from Chlamys farreri (PCF)’s anti-apoptotic effect, HaCaT cells were exposed to 20 mJ/CM2 UVB, with or without pretreatment TGF-β1 antagonist SB431542, inducible nitric oxide synthase (iNOS) inhibitor S-methylisothiourea sulfate (SMT), scavenger carboxy-PTIO, 1.42, 2.84, and 5.69 mM PCF, iNOS transfection (without UVB exposure). Apoptosis was confirmed Hoechst 33258 staining; RT-PCR western blot used determine expression levels signaling pathway. Both exposure transfection-induced apoptosis in UVB-exposed HaCat cells, while SMT, carboxy-PTIO all inhibited UVB-induced apoptosis. TGF-β1, Smad4, Smad7 mRNA altered, similarly, iNOS, pSmad2/3 protein altered cells. In pretreated 1.42–5.69 PCF Moreover, treatment pSmad2/3, increased level Smad7. SB431542 did not significantly alter expression, SMT level. The effect involves inhibition subsequently