Molecular mechanism of polypeptides from Chlamys farreri (PCF)’s anti-apoptotic effect in UVA-exposed HaCaT cells involves HSF1/HSP70, JNK, XO, iNOS and NO/ROS
作者： Xiaowen Wang , Qixiao Jiang , Wencheng Wang , Li Su , Yantao Han
DOI: 10.1016/J.JPHOTOBIOL.2013.11.005
关键词: c-Jun N-terminal kinases 、 HSF1 、 Nitric oxide 、 Nitric oxide synthase 、 Hsp70 、 HaCaT 、 Biology 、 Molecular biology 、 Apoptosis 、 Reactive oxygen species 、 Biophysics 、 Radiological and Ultrasound Technology 、 Radiation 、 Radiology Nuclear Medicine and imaging
摘要: This study investigated the molecular mechanisms of polypeptides from Chlamys farreri (PCF)'s anti-apoptotic effects in ultraviolet A-rays (UVA) exposed HaCaT cells. UVA-induced apoptosis cells was confirmed with Hoechst 33258 fluorescent staining; PCF treatment inhibited cells, increased transcriptional activities heat shock factor protein 1 (HSF1) and expression 70 (HSP70), whereas activation c-Jun N-terminal kinases (JNK), xanthine oxidese (XO), inducible nitric oxide synthase (iNOS) release (NO)/reactive oxygen species (ROS). Meanwhile, HSF1 transcription inhibitor quercetin apoptosis, JNK, XO iNOS NO/ROS. Among two NO peaks we found UVA oxypurinol to be able inhibit at 3h post exposure but not 18h, while S-methylisothiourea sulfate (SMT) 18h 3h. PCF's protection against involves activity HSF1, HSP70, subsequential inhibition JNK pathway, ROS/NO release.
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