Ursolic acid induced anti-proliferation effects in rat primary vascular smooth muscle cells is associated with inhibition of microRNA-21 and subsequent PTEN/PI3K.
作者： Qixiao Jiang , Yantao Han , Hui Gao , Rong Tian , Ping Li
DOI: 10.1016/J.EJPHAR.2016.04.001
关键词:
摘要: This study focused on the anti-proliferation effects of ursolic acid (UA) in rat primary vascular smooth muscle cells (VSMCs) and investigated underlying molecular mechanism action. Rat VSMCs were pretreated with UA (10, 20 or 30μM) amino guanidine (AG, 50μM) for 12h PI3K inhibitor LY294002 30min Akt MK2206 24h, then 10% fetal bovine serum was used to induce proliferation. CCK-8 assess cell To explore mechanism, treated 30μM), MK2206, transient transfected inhibit miRNA-21 (miRNA-21) overexpress PTEN, quantitative real-time PCR mRNA levels phosphatase tensin homolog (PTEN) inhibitor; western blotting measure protein PTEN PI3K. exerted significant VSMCs. Furthermore, inhibited expression subsequently enhanced PTEN. found In conclusion, exerts VSMCs, which is associated inhibition modulation PTEN/PI3K signaling pathway.
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