Expression Profiles of Endometrial Leukemia Inhibitory Factor, Transforming Growth Factor β2 (TGFβ2), and TGFβ2 Receptor in Infertile Bonnet Monkeys1

摘要: The expression profiles of leukemia inhibitory factor (LIF), transforming growth β2 (TGFβ2), and receptor (TGFβ2R) were analyzed during the peri-implantation period in regularly menstruating, fertile bonnet monkeys animals which endometrial nonreceptivity was induced by administering an antiprogestin, onapristone. Based on our previous experiences, a dose 2.5 or 5 mg onapristone administered s.c. every third day menstrual cycle, because these dosages impair development without upsetting normal gonadal endocrine profiles. Endometrial biopsy specimens collected proliferative phase (estradiol levels about 200 pg/ml, n = 5) (Day 8 after midcycle peak estradiol levels, from ovulatory onapristone-treated (n 10). processed to determine patterns LIF, TGFβ2, TGFβ2R immunohistochemical reverse transcription-polymerase chain reaction (RT-PCR) methods. Levels both protein mRNA for (analyzed immunohistochemistry RT-PCR, respectively) greater samples compared control animals. Treatment with either two doses (2.5 mg) caused significant (P < 0.05) down-regulation LIF endometria. expressions TGFβ2 mRNAs reduced significantly treated onapristone, but not those lower dose. However, immunoreactive proteins down-regulated 2.5- 5-mg-treated groups. alterations observed specific, epidermal remained unaffected endometria present study demonstrates derangement infertile monkeys. It may be hypothesized that function is one early steps regulation progesterone-driven cascade events leading receptivity, any aberration this step adversely affect subsequent molecular (i.e., LIF). These data also suggest potential aberrations functional network locally produced cytokines factors even occur endometrium exposed optimal peripheral hormonal levels.