Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq).
作者： Yuanyuan Zheng , Taiyu Hui , Chang Yue , Jiaming Sun , Dan Guo
DOI: 10.1038/S41598-019-57404-9
关键词: Non-coding RNA 、 RNA-Seq 、 Computational biology 、 Circular RNA 、 Cashmere goat 、 Alternative splicing 、 Biology 、 Differential expression 、 RNA 、 Inner mongolia
摘要: Circular RNA (circRNA) is endogenous non-coding (ncRNA) with a covalently closed circular structure. It mainly generated through alternative splicing or back-splicing. CircRNA known in the majority of eukaryotes and very stable. However, knowledge circRNA involved regulating cashmere fineness limited. Skin samples were collected from Liaoning goats (LCG) Inner Mongolia (MCG) during anagen period. For differentially expressed circRNAs, sequencing was performed, analysis led to an identification 17 up-regulated circRNAs 15 down-regulated LCG compared MCG skin samples. In order find LCG, we carried out qPCRs on 10 candidate coarse type (CT-LCG) fine (FT-LCG). Four circRNAs: ciRNA128, circRNA6854, circRNA4154 circRNA3620 confirmed be significantly differential expression LCG. Also, regulatory network circRNAs-miRNAs bioinformatically deduced may help understand molecular mechanisms potential involvement fineness.
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