Characterization of Liaoning Cashmere Goat Transcriptome: Sequencing, De Novo Assembly, Functional Annotation and Comparative Analysis

摘要: Background Liaoning cashmere goat is a famous breed for wool. In order to increase the transcriptome data and accelerate genetic improvement this breed, we performed de novo sequencing generate first expressed sequence tag dataset Liaoning goat, using next-generation technology. Results Transcriptome of on Roche 454 platform yielded 804,601 high-quality reads. Clustering assembly these reads produced non-redundant set 117,854 unigenes, comprising 13,194 isotigs 104,660 singletons. Based similarity searches with known proteins, 17,356 unigenes were assigned 6,700 GO categories, terms summarized into three main categories 59 sub-categories. 3,548 46,778 had significant existing sequences in KEGG COG databases, respectively. Comparative analysis revealed that 42,254 aligned 17,532 different NCBI nucleotide databases. 97,236 (82.51%) mapped 30 chromosomes. 35,551 (30.17%) matched 11,438 reported protein-coding genes. The remaining non-matched further compared cattle human reference genes, 67 putative new genes discovered. Additionally, 2,781 potential simple repeats initially identified from all unigenes. Conclusion The was deep sequenced, assembled, annotated, providing abundant better understand transcriptome. provide material basis future linkage quantitative trait loci analyses.