Distinct aspects of neuronal differentiation encoded by frequency of spontaneous Ca2+ transients.

摘要: STIMULATION of transient increases in intracellular calcium (Cai2+) activates protein kinases1–3, regulates transcription4–9 and influences motility morphology10–12. Developing neurons generate spontaneous Cai2+ transients, but their role directing neuronal differentiation the way which they encode information are unknown. Here we image Ca2+ spinal throughout an extended period early development, find that two types events, spikes waves, expressed at distinct frequencies. Neuronal is altered when eliminated by preventing influx. Reimposing different frequency patterns elevation demonstrates natural spike activity sufficient to promote normal neurotransmitter expression channel maturation, whereas wave regulate neurite extension. Suppression elevations BAPTA loaded intracellularly indicates also necessary for differentiation. transients appear frequency, like action potentials, although 104 times longer duration less frequent, implement intrinsic development programme.