Detection of betanodavirus in juvenile barramundi, Lates calcarifer (Bloch), by antigen capture ELISA.
作者： B J Fenner , Q Du , W Goh , R Thiagarajan , H K Chua
DOI: 10.1111/J.1365-2761.2006.00736.X
关键词: Epitope mapping 、 Virology 、 Biology 、 Molecular biology 、 Viral nucleocapsid 、 Betanodavirus 、 Recombinant DNA 、 Antigen 、 Monoclonal antibody 、 Antibody 、 Virus
摘要: Betanodavirus infection of fish has been responsible for mass mortalities in aquaculture hatcheries worldwide. Betanodaviruses possess a bipartite single-stranded RNA genome consisting the 3.1 kb RNA1 encoding an RNA-dependent polymerase and B2 protein, while 1.4 RNA2 encodes viral nucleocapsid alpha. A panel six monoclonal antibodies against alpha protein greasy grouper nervous necrosis virus (GGNNV) was developed use diagnostics. All reacted with native recombinant immunoblot indirect immunofluorescence assays. Each discrete regions though none extreme C-terminal region protein. One antibodies, specific K151-T246 alpha, used development antigen capture ELISA. In this assay we could detect 10(3)-10(4) TCID(50) units derived from infected tissue culture supernatants. Head extracts prepared experimentally barramundi, Lates calcarifer, juveniles were assayed GGNNV using clear increase detected 5 to 15 days post-challenge. The thus represents useful method field-based detection betanodavirus hatcheries.
core.ac.uk
researchgate.net 
sci-hub.se 参考文章(22)
B.L. Munday, T. Nakai, Nodaviruses as pathogens in larval and juvenile marine finfish World Journal of Microbiology & Biotechnology. ,vol. 13, pp. 375- 381 ,(1997) , 10.1023/A:1018516014782
SC Chi, JR Shieh, SJ Lin, Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Diseases of Aquatic Organisms. ,vol. 55, pp. 221- 228 ,(2003) , 10.3354/DAO055221
Huang, Tan, Chang, Munday, Mathew, Ngoh, Kwang, Detection of nodavirus in barramundi, Lates calcarifer (Bloch), using recombinant coat protein‐based ELISA and RT–PCR Journal of Fish Diseases. ,vol. 24, pp. 135- 141 ,(2001) , 10.1046/J.1365-2761.2001.00270.X
Y LAI, In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus Vaccine. ,vol. 20, pp. 3221- 3229 ,(2002) , 10.1016/S0264-410X(02)00239-6
A. Hegde, H. C. Teh, T. J. Lam, Y. M. Sin, Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata--comparative characterization and pathogenicity studies. Archives of Virology. ,vol. 148, pp. 575- 586 ,(2003) , 10.1007/S00705-002-0936-X
L DALLAVALLE, V TOFFOLO, M LAMPRECHT, C MALTESE, G BOVO, P BELVEDERE, L COLOMBO, Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR Veterinary Microbiology. ,vol. 110, pp. 167- 179 ,(2005) , 10.1016/J.VETMIC.2005.07.014
Ingunn Sommerset, Rasmus Skern, Eirik Biering, Hogne Bleie, Ingrid Uglenes Fiksdal, Søren Grove, Audun Helge Nerland, Protection against Atlantic halibut nodavirus in turbot is induced by recombinant capsid protein vaccination but not following DNA vaccination Fish & Shellfish Immunology. ,vol. 18, pp. 13- 29 ,(2005) , 10.1016/J.FSI.2004.03.006
Jean-Luc Coeurdacier, Florent Laporte, Jean-François Pepin, Preliminary approach to find synthetic peptides from nodavirus capsid potentially protective against sea bass viral encephalopathy and retinopathy. Fish & Shellfish Immunology. ,vol. 14, pp. 435- 447 ,(2003) , 10.1006/FSIM.2002.0449
Claude M Fauquet, M Aꎬ Mayo, Jack Maniloff, Ulrich Desselberger, Laurence Andrew Ball, None, Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press. ,(2005)
JR Shieh, SC Chi, Production of monoclonal antibodies against grouper nervous necrosis virus (GNNV) and development of an antigen capture ELISA Diseases of Aquatic Organisms. ,vol. 63, pp. 53- 60 ,(2005) , 10.3354/DAO063053