Sonic hedgehog-mediated ventralization disrupts formation of the midbrain-hindbrain junction in the chick embryo.

作者: Xiang-Mei Zhang , Eddie Lin , Xian-Jie Yang

DOI: 10.1159/000017443

关键词: HindbrainMesodermNeural tubeCentral nervous systemBiologyNogginNeuroscienceMidbrainSonic hedgehogNeurulation

摘要: The secreted signaling molecule Sonic hedgehog (Shh) plays critical roles in pattern formation of the vertebrate central nervous system. During neurulation, Shh is produced by ventral midline mesoderm as well neural tube, and its activity required for determination characteristics along anterior-posterior neuraxis. morphological boundary between midbrain hindbrain, isthmus, an important tissue organizer that regulates development both anterior hindbrain. In this study, we report retrovirus-mediated misexpression early chick tube disrupts causes a fusion bilateral cerebellum primordia. Dorsally expressed signals induce ectopic transcription receptor Ptc1 expression several genes (Noggin, Lmx1, BMP7) dorsal abolished, or lateral markers (HNF3β, Ptc1, ELF1) are induced brain. Furthermore, normally restricted two (En1 Pax2) mid/hindbrain junction region expanded, reflecting defects. These results suggest maintaining proper dorsal-ventral patterns essential normal region.

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