Chapter 10 Manipulating Gene Expression with Replication--Competent Retroviruses

摘要: Publisher Summary The obvious advantages of the chick for embryological studies have been tempered by difficulties genetic analyses in this organism. ability to manipulate gene expression mouse embryo has rendered it organism choice studies, but difficulty physically manipulating mammalian limits combination these approaches study development. use retroviral vectors alter proven be a valuable alternative transgenic approaches. Many manipulations that so powerful can mimicked with vectors. retroviruses several stemming from comparative ease which different patterns may generated same virus. genes are reused tissues and at times mediate By varying infection protocol, single high titer virus stock used address role many developmental decisions.