Diversidad molecular de begomovirus en el cultivo del pimiento (Capsicum annuum L.) en Cuba
作者： Francisco Murilo Zerbini , Gloria Castillo Urquiza , Madelaine Quiñones Pantoja , Poliane Alfenas Zerbini
DOI:
关键词: Veterinary medicine 、 HindIII 、 Restriction fragment length polymorphism 、 Begomovirus 、 Cartography 、 Pepper 、 Biology 、 Genome amplification 、 Rolling circle replication
摘要: The main areas for pepper production in Cuba were surveyed during 2006-2010 to determine the molecular diversity of begomoviruses this crop. Six hundred leaf samples with typical symptoms viral diseases collected, and from them, 50 per region randomly selected their evaluation. total DNA extracted each sample was previously tested by conventional PCR using begomovirus universal primers (Palv 1978/PARc496). positive used as a template full-length circular genome amplification Rolling Circle Amplification. amplified products characterized restriction fragment length polymorphism analysis (RFLP) enzymes Sac I, BamH Hind III, Cla Kpn Sal Pst I.Begomovirus infection detected 100% evaluated PCR, suggesting presence these viruses all regions country. Bamh 1 Investigacion financiada por la agencia Brasilera CAPES (Brasil), en el marco del proyecto de investigacion CAPES/MESCuba 030/-07 y apoyada 00300281 PNCT Biotecnologia Agropecuaria (Cuba).
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