Endotoxin and lipid A stimulate proliferation of human T cells in the presence of autologous monocytes.

摘要: In this paper we describe a new activity of LPS and partial structures: the induction DNA synthesis lymphokine production human T lymphocytes. The LPS-induced cell proliferation is dose dependent requires 100 to 10,000 ng/ml or synthetic lipid A (compound 506) for optimal stimulation. contrast, precursor Ia 406) not active but rather antagonizes proliferation. accompanied by expression mRNA Th1 cell-derived lymphokines IFN-gamma IL-2, Th2 IL-4, IL-5, IL-10. Highly enriched lymphocyte preparations with less than 0.1% monocytes are stimulated LPS, showing that required Reconstitution experiments show only monocytes, B lymphocytes, able support synthesis. Separating LPS-stimulated from lymphocytes membrane, permeable cytokines cells, abolishes Fixation paraformaldehyde also abrogates their accessory function cells. If preincubated 2 h at 37 degrees C then washed, they still induce in absence additional LPS. Our results indicate cells respond monocyte-supported manner exposure production. We hypothesize reactivity may be clinical relevance.