Metabolite profiling of carotenoid and phenolic pathways in mutant and transgenic lines of tomato: identification of a high antioxidant fruit line.

摘要: Plant secondary metabolism is highly regulated within the major pathways to terpenoids, phenolics and alkaloids. Such regulation can occur at multiple levels from transcription through compartmentation of product. However, possibility exists for cross-talk between these pathways, which largely unknown present. phenomena are important understand in application plant breeding, where unintended effects transgenesis or mutation have an impact on environment human health. In effort improve dietary antioxidant content crop plants, tomato has been a focus engineering both lipophilic antioxidants such as carotenoids hydrophilic flavonoid glycosides. this study, panel transgenic mutant lines subjected metabolite profiling comparison with wild type Ailsa Craig phenolics. A range mutants were selected showing phenotypes varying down-regulation increased lycopene β-carotene. All altered structural genes carotenoid biosynthesis showed that perturbations do not generally alter phenolic flavonoids significantly even when devoid carotenoids. Reciprocally, ferulate 5-hydroxylase had no effect content. contrast defective light perception high pigment (hp-1) LA3771 possess elevated chlorogenic acid rutin well These act hosts further genetic manipulation