miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer.
作者： Yuanqiang Lin , Kailiang Cheng , Tongtong Wang , Qian Xie , Minglong Chen
DOI: 10.1016/J.BIOPHA.2017.09.074
关键词: Cell culture 、 Real-time polymerase chain reaction 、 microRNA 、 Cell growth 、 Apoptosis 、 Thyroid cancer 、 Downregulation and upregulation 、 Cancer research 、 Biology 、 In vivo
摘要: Abstract Purpose The aims of this study were to test the influence miR-217 on proliferation, invasion, migration thyroid cancer and relevant mechanism. Method expression levels in tissues cell lines detected by quantitative real-time PCR (qRT-PCR).Cell Counting Kit-8, flow cytometer, wound healing, transwell invasion assays applied evaluate effect apoptosis, cells. luciferase reporter assay, qRT-PCR, western blot used identify target miR-217. Relative relationship level between AKT3 was analyzed tissues. Xenograft transplantation performed vivo . Results We found that significantly decreased lines. Significantly, associated with clinical stage lymph node metastasis. Function studies revealed overexpression cells inhibited migration, vitro , as well suppressed tumor growth Subsequently, identified a cancer. upregulated tissues, inversely correlated miR-217expression. Besides, efficiently abrogates suppressive caused Conclusion These data demonstrated suppressor role for development progression targeting AKT3, suggesting might be potential
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