Construction of an integrative regulatory element and variation map of the murine Tst locus.

摘要: Given the abundance of new genomic projects and gene annotations, researchers trying to pinpoint causal genetic variants are faced with a challenging task how efficiently integrate all current information. The objective study was develop an approach various annotations for recently positionally-cloned Tst (Thiosulfate Sulfur Transferase, synonym Rhodanese) responsible Fob3b2 QTL effect on leanness improved metabolic parameters. second aim identify prioritize that may be phenotypic effects. A bioinformatics developed existing knowledge regulatory elements gene. entire locus along flanking segments sequenced between our unique polygenic mouse Fat Lean strains were generated by divergent selection adiposity over 60 generations. bioinformatics-generated element map then combined mice comparative analyses polymorphisms across 17 in order prioritise likely polymorphisms. Two candidate identified, one overlapping evolutionary constrained intronic other residing seed region predicted 3′UTR miRNA binding site. This identified increased likelihood. provides basis experimental validation functional this novel antidiabetic Our methodological is general utility analyzing regulation loci have limited evidence identifying post-GWAS or post-QTL- cloning studies.