Mast cells infiltrate the esophageal smooth muscle in patients with eosinophilic esophagitis, express TGF-β1, and increase esophageal smooth muscle contraction

摘要: Background Increased numbers of mast cells are present in the esophageal epithelium patients with eosinophilic esophagitis (EE). However, cell infiltration into lamina propria (LP) and smooth muscle (SM) effects their products on SM function has not been determined. Objective We investigated localization characterization SM, functional significance TGF-β1 expression to contraction human (HESM) vitro , effect topical corticosteroids number tryptase-positive (MC T ) chymase-positive C EE. Methods MC - -positive were quantitated epithelium, LP before after corticosteroid therapy, muscularis mucosa EE control subjects by using immunohistology. Double immunofluorescence was used assess production TGF-β1. The ability influence HESM contractility assessed . Results In EE, significantly increased TGF-β1–positive (but only low levels eosinophils) detected compared those seen subjects. expressed TGF-β1, which cultured primary Topical therapy reduced epithelial but tryptase-chymase–positive numbers. Conclusions numbers, rather than eosinophil express increase As such, localized might modulate contractility.