作者: Zekeriya Biyiklioglu , Arzu Özel , Burak Barut , Turgut Keleş
DOI: 10.1016/J.BIOORG.2021.104637
关键词: Cancer cell 、 Cytotoxicity 、 Chemistry 、 Agarose gel electrophoresis 、 Molecular biology 、 Cell growth 、 MTT assay 、 Nuclease 、 A549 cell 、 Topoisomerase
摘要: Abstract Cancer has become an important public problem in worldwide since cancer incidence and mortality are growing rapidly. In this study, water soluble non-aggregated silicon (IV) phthalocyanines naphthalocyanines containing (3,5-bis{3-[3-(diethylamino)phenoxy]propoxy}phenyl)methoxy groups have been synthesized characterized to investigate their anticancer potential. Their DNA binding/nuclease, topoisomerases inhibition were investigated using UV–Vis absorption, thermal denaturation agarose gel electrophoresis. The vitro cytotoxic properties of the compounds on human lung (A549), breast (BT-20), liver (SNU-398), prostate (DU-145), melanoma (SK-Mel 128) carcinoma fibroblast (HFC) normal cell lines evaluated by MTT assay. order determine mechanism growth suppression, cycle analysis was carried out flow cytometer A549 line. Kb values SiPc1a SiNc2a 6.85 ± (0.35) × 106 1.72 ± (0.16) × 104 M−1 Tm ct-DNA calculated as 82.02 °C 78.07 °C, respectively presence both compounds. ΔTm 6.45 2.50 °C, respectively. nuclease effects with supercoiled plasmid pBR322 demonstrated that did not trigger any at lowest concentrations without irradiation whereas activating agent (H2O2) showed significant actions under (22.5 J/cm2). inhibited topoisomerase I increasing whilst they had lower action toward II I. cytotoxicity studies displayed highest among tested against A549, SNU-398, SK-MEL128, DU-145, BT-20 HFC CC50 ranged from 0.49 2.99 µM. Furthermore, proliferation arrest G0/G1 phase. All these results suggested is a promising candidate agent.