Persistent growth impairment of bone marrow stroma after antilymphocyte globulin treatment for severe aplastic anaemia and its association with relapse.

摘要: :  Bone marrow from 65 patients with aplastic anaemia (AA) was tested for stroma growth in short term cultures (2 weeks) and colony formation by haemopoietic precursor cells during the course of their disease. In 18 untreated patients, mean 30% normal virtually absent. After treatment immunosuppression (IS), as estimated 90 examinations 54 approximately 50% 10% normal. Growth impairment precursors persisted 10 more years after IS. Results 2-week were compared results long bone AA 4 controls. At 2 weeks, delayed to normal, but this difference became less evident prolonged incubation time. vitro abnormalities clinical evolution The development late haematological complications (paroxysmal nocturnal haemoglobinuria (PNH)) myelodysplastic syndrome (MDS), did not correlate degree impairment. However, relapse aplasia associated poor growth: 8/29 confluence ≤30% remission versus 1/25 >30% relapsed. We conclude that (i) haematopoietic microenvironment is frequently coinvolved disease process AA, (ii) a defect detected rather than and, (iii) frequent growth.