Amperometric biosensor for glyphosate based on the inhibition of tyrosinase conjugated to carbon nano-onions in a chitosan matrix on a screen-printed electrode.
作者： Vibol Sok , Alex Fragoso
DOI: 10.1007/S00604-019-3672-6
关键词: Substrate (chemistry) 、 Matrix (chemical analysis) 、 Chitosan 、 Carbon 、 Glyphosate 、 Detection limit 、 Chemistry 、 Biosensor 、 Chromatography 、 Tyrosinase
摘要: Glyphosate [N-(phosphonomethyl)glycine] is the most frequently used herbicide to date. Due its indiscriminate use, it has become a globally occurring pollutant of surface waters. A biosensor for glyphosate described here that consists carbon nano-onion/tyrosinase conjugate immobilized in chitosan matrix on screen-printed electrode. The analytical principle based inhibition enzyme tyrosinase by glyphosate. L-DOPA as substrate. presence nano-onions beneficial effect sensitivity assay. can be amperometrically quantified 0.015 10 μM concentration range and with 6.5 nM (1.1 μg L−1) detection limit. stable more than 2 months at 4 °C. It was applied water and soil samples taken from irrigation rice field after aerial application. Results were good agreement data obtained commercial ELISA.
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