Identification of Southeastern Bat Species Using Noninvasive Genetic Sampling of Individual Guano Pellets
作者： Veronica A. Brown , Emma V. Willcox , Kirstin E. Fagan , Riley F. Bernard
关键词: National park 、 Ribosomal RNA 、 Netting 、 Guano 、 Mitochondrial DNA 、 Biology 、 Ecology 、 Genetic analysis 、 Feces 、 Genetic marker
摘要: Abstract The impact of white-nose syndrome on North American bat populations may limit the effectiveness traditional monitoring methods, including roost surveys, mist netting, and acoustic monitoring, and, in turn, determination species occurrence. Genetic markers from deoxyribonucleic acid (DNA) extracted feces (i.e., guano pellets) provide an effective alternative method for assessing We used existing genetic marker 16S ribosomal subunit, mitochondrial DNA, to create a DNA sequence database 16 bats known occur Tennessee. our identify 68 pellets collected accumulations found buildings Great Smoky Mountains National Park May August 2015. No were directly observed at 19 (55.9% all identified buildings), where analysis was only available determine Two we detected roo...
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