Development of an in vitro assay and demonstration of Plasmodium berghei liver-stage inhibition by TRAP-specific CD8+ T cells.
作者： Rhea J. Longley , Karolis Bauza , Katie J. Ewer , Adrian V. S. Hill , Alexandra J. Spencer
DOI: 10.1371/JOURNAL.PONE.0119880
关键词: Biology 、 Cell culture 、 CD8 、 Cytotoxic T cell 、 T cell 、 Immunity 、 Plasmodium berghei 、 Antigen 、 Virology 、 Molecular biology 、 In vitro
摘要: The development of an efficacious vaccine against the Plasmodium parasite remains a top priority. Previous research has demonstrated ability prime-boost virally vectored sub-unit vaccination regimen, delivering liver-stage expressed malaria antigen TRAP, to produce high levels antigen-specific T cells. is main target cell-mediated immunity, yet major challenge in assessing new cell inducing vaccines been lack suitable pre-clinical assay. We have developed flow-cytometry based vitro killing assay using mouse hepatoma line, Hepa1-6, and berghei GFP expressing sporozoites. Using this assay, P. TRAP-specific CD8+ enriched splenocytes were shown inhibit parasites effector-to-target ratio dependent manner. Further human hepatocytes falciparum would provide method pre-clinically screen candidates elucidate mechanisms protection vitro.
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R. S. Nussenzweig, Alan Schwartz, E. H. Nardin, S. Tharavanij, M. R. Hollingdale, Inhibition of entry of Plasmodium falciparum and P. vivax sporozoites into cultured cells: an in vitro assay of protective antibodies Journal of Immunology. ,vol. 132, pp. 909- 913 ,(1984)
M. Leef, S. Hoffman, Y. Charoenvit, C. Paul, S. Mellouk, L. Yuan, M. Sedegah, B. Galey, N. Berbiguier, R. Beaudoin, P. Druilhe, Evaluation of an in vitro assay aimed at measuring protective antibodies against sporozoites. Bulletin of The World Health Organization. ,vol. 68, pp. 52- 59 ,(1990)
J A Berzofsky, S L Hoffman, W R Weiss, R A Houghten, M Hollindale, M Sedegah, A T cell clone directed at the circumsporozoite protein which protects mice against both Plasmodium yoelii and Plasmodium berghei. Journal of Immunology. ,vol. 149, pp. 2103- 2109 ,(1992)
Denise L. Doolan, Stephen L. Hoffman, IL-12 and NK cells are required for antigen-specific adaptive immunity against malaria initiated by CD8+ T cells in the Plasmodium yoelii model. Journal of Immunology. ,vol. 163, pp. 884- 892 ,(1999)
L Rénia, D Grillot, M Marussig, G Corradin, F Miltgen, PH Lambert, D Mazier, G Del Giudice, Effector functions of circumsporozoite peptide-primed CD4+ T cell clones against Plasmodium yoelii liver stages. Journal of Immunology. ,vol. 150, pp. 1471- 1478 ,(1993)
Andrea Crisanti, E Scarselli, Hm Muller, Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum in parasite-host cell interactions. Parassitologia. pp. 69- 72 ,(1993)
S Khusmith, M Sedegah, S L Hoffman, Complete protection against Plasmodium yoelii by adoptive transfer of a CD8+ cytotoxic T-cell clone recognizing sporozoite surface protein 2 Infection and Immunity. ,vol. 62, pp. 2979- 2983 ,(1994) , 10.1128/IAI.62.7.2979-2983.1994
Michael R. Betts, Jason M. Brenchley, David A. Price, Stephen C. De Rosa, Daniel C. Douek, Mario Roederer, Richard A. Koup, Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation Journal of Immunological Methods. ,vol. 281, pp. 65- 78 ,(2003) , 10.1016/S0022-1759(03)00265-5
Perry M. Scanlan, Vaibhav Tiwari, Susmita Bommireddy, Deepak Shukla, Spinoculation of heparan sulfate deficient cells enhances HSV-1 entry, but does not abolish the need for essential glycoproteins in viral fusion Journal of Virological Methods. ,vol. 128, pp. 104- 112 ,(2005) , 10.1016/J.JVIROMET.2005.04.008
Julius Clemence R. Hafalla, Karolis Bauza, Johannes Friesen, Gloria Gonzalez-Aseguinolaza, Adrian V. S. Hill, Kai Matuschewski, Identification of Targets of CD8+ T Cell Responses to Malaria Liver Stages by Genome-wide Epitope Profiling PLoS Pathogens. ,vol. 9, pp. e1003303- ,(2013) , 10.1371/JOURNAL.PPAT.1003303