Use of PCR for Diagnosis of Post-Kala-Azar Dermal Leishmaniasis
作者： Omran F Osman , Linda Oskam , Nel CM Kroon , Gerard J Schoone , El-Tahir AG Khalil
DOI: 10.1128/JCM.36.6.1621-1624.1998
关键词: Leishmaniasis 、 Mycobacterium leprae 、 Post-kala-azar dermal leishmaniasis 、 Bone marrow 、 Lymph node 、 Visceral leishmaniasis 、 Pathology 、 Scars 、 Medicine 、 Lymph
摘要: Microscopy and PCR were compared for use in the diagnosis of post-kala-azar dermal leishmaniasis (PKDL) 63 patients. Aspirates lymph nodes (samples from 52 patients), skin (23 samples), bone marrow (18 samples) used. For 11 patients node aspiration could be repeated 6 months after they recovered PKDL. During active PKDL, was positive 42 (80.8%) aspirates 19 23 (82.7%) aspirates, whereas microscopy only 9 (17.3%) 7 (30.4%) aspirates. always when parasites seen by microscopy. When results obtained with same patient (n = 16) compared, there complete agreement. Bone samples negative 16 both methods 1 patient; one sample positive. confirmed co-occurrence visceral PKDL suspicion this other patient. After recovery, no found microscopy, but 2 (18.2%) still PCR. Thirty controls all to negative, 15 Cross-reactions Mycobacterium leprae ruled out. In conclusion, inguinal or is suitable confirming clinical some patients, are probably preferred because material may leave scars.
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