Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

摘要: BACKGROUND: Earlier detection of transformed cells using target-specific imaging techniques holds great promise. We have developed TAB 004, a monoclonal antibody highly specific to protein sequence accessible in the tumor form MUC1 (tMUC1). present data assessing both specificity and sensitivity 004 vitro genetically engineered mice vivo. METHODS: Polyoma Middle T Antigen were crossed human MUC1.Tg generate MMT mice. In mice, mammary gland hyperplasia is observed between 6 10 weeks age that progresses ductal carcinoma situ by 12 14 adenocarcinoma 18 24 weeks. Approximately 40% these develop metastasis lung other organs with evolution closely mimics breast cancer progression. Tumor progression was monitored (from ages 8 22 weeks) vivo following retro-orbital injections conjugated indocyanine green (TAB-ICG). At euthanasia, tumors normal epithelial tissues collected for further analyses. RESULTS: TAB-ICG injection permitted significantly earlier compared physical examination. Furthermore, administration enabled metastases while sparing recognition epithelia. CONCLUSIONS: The highlight differentiating versus its utility as targeted agent early detection, monitoring response, well potential clinical use drug delivery.