Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model
作者： Cui‐Ling Dong , William R Webb , Qiang Peng , James Z Tang , Nicholas R Forsyth
DOI: 10.1002/JBM.A.35149
关键词: Mesenchymal stem cell 、 Materials science 、 Stem cell 、 Tissue culture 、 Tissue engineering 、 Platelet-derived growth factor 、 Biophysics 、 Matrix (biology) 、 Growth factor 、 Platelet-derived growth factor receptor 、 Biomedical engineering
摘要: This study aimed to design a growth factor loaded copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) nanoparticles containing 3D collagen matrix achieve sustained release for long-term stimulation human mesenchymal stem cells (hMSCs) proliferation/differentiation tissue engineer application. Platelet-derived factor-BB (PDGF-BB), which is known enhance hMSCs proliferation in serum, was selected as model factor, biodegradable PHBHHx chosen be the vehicle. PDGF-BB phospholipid complex encapsulated were fabricated, their effect on investigated via assays CCK-8 live-dead staining inoculated 2D culture plates gel scaffolds, respectively. The resulting spherical stable terms mean particle size, polydispersity index zeta potential before after lyophilization. In vitro revealed with low burst release. Furthermore, released significantly promote both cell monolayer seeded scaffolds serum-free media. Therefore, matrices locally hold promise used engineering.
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