Multi-functional P(3HB) microsphere/45S5 Bioglass-based composite scaffolds for bone tissue engineering.

摘要: Novel multi-functional P(3HB) microsphere/45S5 Bioglass((R))-based composite scaffolds exhibiting potential for drug delivery were developed bone tissue engineering. 45S5 glass-ceramic of high interconnected porosity produced using the foam-replication technique coated with biodegradable microspheres (size < 2 mum) made from poly(3-hydroxybutyrate), P(3HB), Bacillus cereus SPV. A solid-oil-in-water emulsion solvent extraction /evaporation was used to produce these microspheres. simple slurry-dipping method, a 1 wt % suspension in water, dispersed by an ultrasonic bath, coat scaffold, producing uniform microsphere-coating throughout 3D scaffold structure. Compressive strength tests confirmed that slightly enhanced mechanical strength. It also did not inhibit bioactivity when immersed simulated body fluid (SBF) up 4 weeks. The hydroxyapatite (HA) growth rate on microsphere Bioglass((R)) very similar uncoated control sample, qualitatively indicating bioactivity. However, surface topography HA layer affected as shown results obtained white light interferometry. roughness much higher microsphere-coated than samples, after seven days SBF. This feature would facilitate cell attachment and proliferation. Finally, gentamycin successfully encapsulated into demonstrate capability scaffolds. Gentamycin release kinetics determined liquid chromatography-mass spectrometry (LC-MS). slow controlled compared observed fast relatively uncontrolled (without coating). Thus, this unique multifunctional bioactive has enhance provide relevant drugs