Capillary Electrophoretic Separation and Laser-Induced Fluorescence Detection of the Major DNA Adducts of Cisplatin and Carboplatin

摘要: Micellar electrokinetic capillary chromatographic separation of a dansylated mixture normal nucleotides and platinated cross-link adducts d(pGpG) d(pApG) was optimized with baseline resolution. The introduction laser-induced fluorescence (LIF) detector overcame the lack sensitivity characteristic electrophoresis (CE) due to small injection volume short optical path length. CE/LIF able detect 1 adduct/104 nucleotides/mg DNA by postlabeling assay. enrichment adduct, prior dansylation, enhanced detection limit adduct/107 DNA. Calf thymus reacted in vitro cisplatin carboplatin total input drug/nucleotide ratios 0.05 0.5, respectively. A2780 human ovarian carcinoma cells were exposed culture 25 mM for 2 h. incubated drug-free medium 3 h before harvesting. identification modified confirmed cochromatography authentic markers. same 1,2-intrastrand induced both its second-generation drug carboplatin. This report has demonstrated, first time, utility as an analytical tool assaying damage.