Effect of potential oocyte transport protocols on blastocyst rates after intracytoplasmic sperm injection in the horse.

摘要: Summary Reasons for performing study Intracytoplasmic sperm injection (ICSI) is used to produce foals from otherwise infertile mares and stallions with limited stores, but requires expensive equipment technically demanding. Methods transport oocytes ICSI laboratories would allow collection of by the referring veterinarian enable greater application this technique. Objectives This study was conducted evaluate protocols that could be immature maturing ICSI. Study design In vitro experiment. Methods Oocytes were recovered transvaginal ultrasound-guided follicular aspiration either dominant follicles 24 h after deslorelin administration (dominant stimulated follicle [DSF]), or subordinate (immature) at same time. To mimic transport, DSF incubated overnight under differing conditions before ICSI; placed in varying maturation, followed ICSI. The rate blastocyst production compared among treatments. Results Blastocysts produced all groups. Dominant held sealed tubes pre-equilibrated control maturation medium maintained 37°C yielded development equal obtained (70%). similarly a warm passive device poor (10%). Immature one 2 nights modified M199 medium, night commercial embryo holding solution, air room temperature, 35–37% per injected oocyte. Conclusions A commercially available can shipping temperature good resulting rates. Better rates oocyte are oocytes; however, these require maintenance as they already time collection, more sensitive delays. This new, practical information supporting both may wider use procedure.