Holding immature equine oocytes in the absence of meiotic inhibitors: effect on germinal vesicle chromatin and blastocyst development after intracytoplasmic sperm injection.

摘要: Holding immature oocytes before the onset of maturation simplifies oocyte transport and aids in scheduling later manipulations. We report here a method for holding equine absence meiotic inhibitors. In Experiment 1, with expanded cumuli were cultured at 38.2 degrees C medium containing cycloheximide, or held room-temperature M199 Hanks' salts, 16-18 h maturation. Control matured immediately after recovery. Oocytes fertilized by intracytoplasmic sperm injection 4d. Embryo development was not different among treatments. 2, treated as but embryos 7.5d. Blastocyst significantly lower cycloheximide-treated group than controls (7% versus 30%) intermediate (16%). 3, roscovitine, room temperature sealed glass vials mixture 40% Earle's 20% FBS (EH treatment) h, maturation, injection, embryo culture EH treatment higher that roscovitine-treated (34% 12%), from (25%). did mature during (70% germinal vesicle stage 18 holding). Whereas cycloheximide roscovitine reduced subsequent blastocyst formation, these could be modified overnight without adverse affecting developmental competence.