Recently integrated human Alu repeats: finding needles in the haystack.

摘要: Alu elements undergo amplification through retroposition and integration into new locations throughout primate genomes. Over 500,000 reside in the human genome, making identification of newly inserted repeats genomic equivalent finding needles haystack. Here, we present two complementary methods for rapid detection integrated elements. In first approach employ computational biology to mine DNA sequence databases order identify recently The second method is based on an anchor-PCR technique which term Allele-Specific PCR (ASAP). this approach, are selectively amplified from anchored generating a display or 'fingerprint' insertion polymorphisms then detected by comparison fingerprints generated different samples. explore utility these applying them members smallest previously identified subfamily genome termed Ya8. This composed about 50 within genome. Approximately 50% Ya8 family have so that they polymorphic, useful markers study evolution.