Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine.

摘要: Background: Antimicrobial peptides (AMP) play a pivotal role in innate host defense and immune response. The delineation of new MS-based profiling tools, which are able to produce panels AMP the nasal fluid (NF), may be attractive for discovery potential diagnostic markers respiratory disorders. Methods: Swabs collected NF from healthy patients with We used fast procedure based on mesoporous silica particles (MPS) enrich its component combination MALDI-TOF/TOF MS as key tool rapidly analyzing clinical samples. Results: Reproducible peptide fingerprints were generated each subject several detected including (Human Neutrophil Peptides) HNPs, Statherin, Thymosin-β4, Peptide P-D, II-2, β-MSP, SLPI, Lysozyme-C, their proteo-forms. In particular, P-D accurately identified by direct MS/MS sequencing. Examples applicability this shown. obtained before after polypectomy well post-treatment azelastine/fluticasone one case allergic rhinitis. Conclusion: our platform implemented materials capturing wider picture might offer an amazing opportunity studies individual population scales.