Structure characterization of two novel polysaccharides isolated from the spikes of Prunella vulgaris and their anticomplement activities

摘要: Abstract Ethnopharmacological relevance The spikes of Prunella vulgaris have long been used as a traditional Chinese medicine to treat various inflammation-related diseases. aim this study was isolate and characterize homogenous polysaccharides from herb evaluate their anticomplement activity. Materials methods Anticomplement activity-guided fractionation the hot water extract P. performed by DEAE-cellulose size-exclusion chromatography, yielding two homogeneous PW-PS1 PW-PS2. homogeneity, molecular weight, monosaccharide composition linkage were determined in addition other chemical characterizations. activity evaluated expressed 50% hemolytic inhibition concentration through classical pathway (CH50 value) alternative (AP50 value). preliminary mechanism for complement activation cascade also assessed. Results PW-PS2 both branched acidic polysaccharides. composed Ara, Xyl, 4-methoxy-Glc A ratio 1.0: 2.6: 0.8. main linkages sugar residues included terminal β- d -Xylp, 1,4-linked 1,3-linked α- -Arap, 1,3,5-linked 4-methoxy-α- -Glcp A. Rha, Gal, Gal 0.6: 1.3: 1.8: 3.4. between Araf, -Rhap, -Galp, 1,4,6-linked -Galp. inhibited pathways with CH50 values 0.28 0.13 mg/mL, respectively, AP50 0.40 0.35 mg/mL, respectively. Preliminary studies using component-depleted sera showed that acted on C1q, C3, C9 components C2, C5, components. Conclusion Our suggested could be valuable treatment diseases associated excessive system.