Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche

摘要: Since the discovery of epithelial hair follicle stem cells (eHFSCs) in bulge human follicles (HFs) an important quest has started: to define useful markers. In current study, we contribute this by critically evaluating corresponding published immunoreactivity (IR) patterns, and attempting identify markers for situ identification eHFSCs their niche. For this, scalp skin cryosections at least five different individuals were examined, employing standard immunohistology as well increased sensitivity methods. Defined reference areas compared quantitative immunohistochemistry relative intensity specific IR. According our experience, most positive turned out be cytokeratin 15, 19 CD200, but not exclusive, while beta1 integrin Lhx2 IR upregulated keratinocytes. Absent CD34, connexin43 nestin on may exploited negative alpha6 integrin, fibronectin, nidogen, fibrillin-1 latent transforming growth factor (TGF)-beta-binding protein-1 expressed throughout connective tissue sheath HFs. On other hand, tenascin-C was thus constitute a component cell niche These immunophenotyping results shed further light expression patterns claimed follicular 'stem markers' suggest that single marker alone only use limited panel offer reasonable pragmatic compromise identifying situ.