Synthesis, Radiolabelling and In Vitro Imaging of Multifunctional Nanoceramics
作者： Marina Lledos , Vincenzo Mirabello , Sophia Sarpaki , Haobo Ge , Hubert J. Smugowski
关键词: Confocal 、 Surface modification 、 Nanotechnology 、 Biocompatibility 、 Quantum dot 、 Molecular imaging 、 Fluorescence-lifetime imaging microscopy 、 Self-assembly 、 In vitro 、 Materials science
摘要: Molecular imaging has become a powerful technique in preclinical and clinical research aiming towards the diagnosis of many diseases. In this work, we address synthetic challenges achieving lab-scale, batch-to-batch reproducible copper-64- gallium-68-radiolabelled metal nanoparticles (MNPs) for cellular purposes. Composite NPs incorporating magnetic iron oxide cores with luminescent quantum dots were simultaneously encapsulated within thin silica shell, yielding water-dispersible, biocompatible NPs. Scalable surface modification protocols to attach radioisotopes 64Cu (t1/2=12.7 h) 68Ga (t1/2=68 min) high yields are reported, compatible time frame radiolabelling. Confocal fluorescence lifetime studies confirm uptake agents their cytoplasmic localisation prostate cancer (PC-3) cells. Cellular viability assays show that biocompatibility system is improved when fluorophores shell. The functional SiO2 matrix represents an ideal platform incorporation radiolabelling incorporation.
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