Open-Space Forced Swim Model of Depression for Mice

作者: Eric A. Stone , Yan Lin

DOI: 10.1002/0471142301.NS0936S54

关键词: Depression (differential diagnoses)PsychologyAnesthesiaPsychological stressPerseverationExtramuralVideo recordingForced swimDevelopmental psychologyMild stress

摘要: This protocol describes a simplified method for inducing chronic depression-like state in mice that is based on the repeated open-space forced swim originally developed rats. The consists of swimming daily lukewarm water rat tub cages, 15 min/day 4 days, and thereafter once per week. procedure produces progressive decrease distance swum concomitant increase immobility (floating) ∼70% mice, both which persist unaltered weeks. model has predictive, face, construct validity number advantages over previous methods it utilizes very mild stress, short duration, easily standardized. Moreover, since greater area than traditional (Porsolt) can be used to study interactions depressive behavior with behavioral flexibility perseveration.

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